Recent Scientific Papers

Click a topic below:

The Basal Lamina
Recombinant Laminin
Anti-Laminin g2
Glomerular Gene Transfer Therapy
Second Generation MMP-2 Inhibitors
Molecular Basis of Autoimmune Disease

The Basal Lamina

"Type IV collagen of the glomerular basement membrane. Evidence that the chain specificity of network assembly is encoded by the non-collagenous NC1 domains", Boutaud, A., Borze, D.B., Bondar, O., Gunwar, S., Netzer, K.O., Singh, N., Ninomiya, Y., Sado, Y. Noelken, M.E., Hudson, B.G., (2000) J. Biol. Chem. 275, 30716-30724.

"Form and function: the laminin family of heterotrimers", Colognato, H., and Yurchenco, P.D. (2000) Dev Dyn 218. 213-234.

"Laminin polymerization induces a receptor-cytoskeleton network", Colognato, H., Winkelmann, D.A., and Yurchenco, P.D. (1999) J. Cell. Biol. 145, 619-631.

"Role of distinct type IV collagen networks in glomerular development and function," Harvey S.J., Zheng K., Sado Y., Naito I., Ninomiya Y., Jacobs R.M., Hudson BG, Thorner PS (1998) Kidney Int. 54, 1857-1866.

"Glomerular basement membrane. Identification of a novel disulfide-cross-linked network of alpha3, alpha4, and alpha5 chains of type IV collagen and its implications for the pathogenesis of Alport syndrome," Gunwar S., Ballester F., Noelken M.E., Sado Y., Ninomiya Y., Hudson B.G. (1998) J. Biol. Chem. 273, 8767-7587.

"Self-assembly of laminin isoforms", Cheng, Y.S., Champliaud, M.F., Burgeson, R.E., Marinkovich, M.P., Yurchenco, P.D. (1997) J. Biol. Chem. 272, 31525-31532.

"Seminiferous tubule basement membrane. Composition and organization of type IV collagen chains, and the linkage of alpha3(IV) and alpha5(IV) chains," Kahsai T.Z., Enders G.C., Gunwar S., Brunmark C., Wieslander J., Kalluri R., Zhou J., Noelken M.E., Hudson B.G. (1997) J. Biol. Chem. 272, 17023-17032.

" Evidence for the existence of multiple heparan sulfate proteoglycans in the human glomerular basement membrane and mesangial matrix," Groffen A.J., Hop F.W., Tryggvason K., Dijkman H., Assmann K.J., Veerkamp J.H., Monnens L.A., Van den Heuvel L.P. (1997) Eur. J. Biochem. 247, 175-182.

"Basal lamina assembly," Yurchenco, P.D. and O'Rear, J.J. (1994) Curr. Opinon Cell Biol. 6, 674-681.

"Type IV collagen: structure, gene organization, and role in human diseases. Molecular basis of Goodpasture and Alport syndromes and diffuse leiomyomatosis," Hudson, B.G., Reeders, S.T. and Tryggvason, K. (1993) J. Biol. Chem. 268, 26033-26036.

"Biochemistry and genetic diseases of glomerular basement membrane," Tryggvason, K. (1993) Seminars Nephrol. 13, 447-456.

"The laminin family," Tryggvason, K. (1993) Curr. Opinion Cell Biol. 5, 877-882.

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"Pyridoxamine, an inhibitor of advanced glycation reactions, also inhibits advanced lipoxidation reactions. Mechanism of action of pyridoxamine", Onorato, J.M., Jenkins, A.J., Thorpe, S.R., Baynes, J.W. (2000) J. Biol. Chem. 275, 21177-21184.

"Amadorins: Novel post-Amadori inhibitors of advanced glycation reactions," Khalifah, R.G., Baynes, J.W., Hudson, B.G. (1999) Biochem. Biophys. Res. Comm. 257, 251-258.

"Alterations in nonenzymatic biochemistry in uremia: orgin and significance of "carbonyl stress" in long-term uremic complications", Miyata, R., van Ypersele de Strihou, C., Kurokawa, K., Baynes, J.W. (1999) Kidney Int., 55, 389-399.

"Pyridorin, a post-Amadori inhibitor of advanced glycation reactions, preserves renal function in diabetic rats," Baynes, J.W., Degenhardt, T.P., Alderson, N.L., and Thorpe, S.R. (1998) J. Am. Soc. Nephrol. 9, 628A.

"Effects of pyridoxamine, a novel post-Amadori AGE inhibitor, on nephropathy induced by glycated albumin," Khalifah, R.G., Booth, A.A., Gattone, V.H., and van den Born, J. (1997) J. Am. Soc. Nephrol. 8, 641A.

"In vitro kinetic studies of formation of antigenic advanced glycation end products (AGEs) - Novel inhibition of post-Amadori glycation pathways," Booth, A.A., Khalifah, R.G., Todd, P. and Hudson, B.G. (1997) J. Biol. Chem. 272, 5430-5437.

"Nonenzymatic glycation of type IV collagen and matrix metalloproteinase susceptibility," Mott, J.D., Khalifah, R.G., Nagase, H., Shield III, C.R., Hudson, J.K., and Hudson, B.G. (1997) Kidney Intern. 52, 1302-1312.

"Kinetics of nonenzymatic glycation of ribonuclease A leading to advanced glycation end products. Paradoxical inhibition by ribose leads to facile isolation of protein intermediate for rapid post-Amadori studies," Khalifah, R.G., Todd, P., Booth, A.A., Yang, S.X., Mott, J.D. and Hudson, B.G. (1996) Biochemistry 35, 4645-4654.

"Pathways of formation of glycoxidation products during glycation of collagen," Wells-Knecht, M.C., Thorpe, S.R. and Baynes, J.W. (1995) Biochemistry 34, 15134-15141.

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"New functions for non-collagenous domains of human collagen type IV. Novel integrin ligands inhibiting angiogenesis and tumor growth in vivo", Petitclerc, E., Boutaud, A., Prestayko, A., Xu, J., Sado, Y., Ninomiya, Y., Sarras, M.P.Jr., Hudson, B.G., and Brooks, P.C. (2000) J. Biol. Chem. 275, 8051-8061.

"Type IV collagen of the glomerular basement membrane. Evidence that the chain specificity of network assembly is encoded by the non-collagenous NC1 domains", Boutaud, A., Borze, D.B., Bondar, O., Gunwar, S., Netzer, K.O., Singh, N., Ninomiya, Y., Sado, Y. Noelken, M.E., Hudons, B.G., (2000) J. Biol. Chem. 275, 30716-30724.

"Comparative analysis of the non-collagenous NC1 domain of type IV collagen: Identification of structural features important for assembly: function and pathogenesis," Netzer, K.-O., Suzuki, K., Itoh, Y., Hudson, B.G. and Khalifah, R.G. (1997) Prot. Sci. 7, 1340-1351.

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Recombinant Laminin

"Recombinant laminin-8 (alpha(4)beta(1)gamma(1)). Production, purification, and interactions with integrins", Kortesmaa, J., Yurchenco, P., Tryggvason, K. (2000) J. Biol. Chem. 275, 14853-14859.

"Molecular cloning and tissue-specific expression of a novel murine laminin gamma3 chain," Iivanainen A., Morita T., Tryggvason K. (1999) J. Biol. Chem. 274, 14107-14111.

"PCR based mutation screening of the laminin alpha2 chain gene (LAMA2): application to prenatal diagnosis and search for founder effects in congenital muscular dystrophy," Guicheney P., Vignier N., Zhang X., He Y., Cruaud C., Frey V., Helbling-Leclerc A., Richard P., Estournet B., Merlini L., Topaloglu H., Mora M., Harpey J.P., Haenggeli C.A., Barois A., Hainque B., Schwartz K., Tome F.M., Fardeau M., Tryggvason K. (1998) J Med. Genet. 35, 211-217.

"The a chain of laminin-1 is independently secreted and drives the secretion of its b- and g- chain partners," Yurchenco, P.D., Quan, Y., Colognato, H., Mathus, T., Harrison, D., Yamada, Y., and O'Rear, J.J. (1997) Proc. Natl. Acad. Sci. USA 94, 10189.

"Primary structure, developmental expression, and immunolocalization of the murine laminin a4 chain," Iivanainen, A., Kortesmaa, Sahlberg, C., Morita, T., Bergmann, U., Thesleff, I., and Tryggvason, K. (1997) J. Biol. Chem. 272, 27862.

"Primary structure and expression of a novel human laminin a4 chain," Iivanainen, A., Sainio, K., Sariola, H. and Tryggvason, K. (1995) FEBS Lett. 365, 183-188.

"Human laminin M chain (merosin): Complete primary structure, chromosomal assignment and expression of the M and A chain in human fetal tissues," Vuolteenaho, R., Nissinen, M., Sainio, K., Byers, M., Eddy, R., Hirvonen, H., Shows, T.B., Sariola, H., Engvall, E. and Tryggvason, K. (1994) J. Cell. Biol. 124, 381-394.

"Primary structure of the human laminin A chain: Limited expression in human tissues," Nissinen, M., Vuolteenaho, R., Boot-Handford, R., Kallunki, P. and Tryggvason, K. (1991) Biochem. J. 276, 369-379.

"Human laminin b2 chain: Comparison of the complete amino acid sequence with the b1 chain reveals variability in sequence homology between different structural domains, " Pikkarainen, T., Kallunki, T. and Tryggvason, K. (1988) J. Biol. Chem. 263, 6751-6758.

"Human laminin b1 chain: A multidomain protein with gene (LAMB1) locus in the q22 region of chromosome 7" Pikkarainen, T., Eddy, R., Fukushima, Y., Byers, M., Shows, T., Pihlajaniemi, T., Saraste, M. and Tryggvason, K. (1987) J. Biol. Chem. 262, 10454-10462.

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Anti-Laminin g2

"Laminin-5 as a marker of invasiveness in cervical lesions", Skyldberg, B., Salo, S., Eriksson, E., Aspenblad, U., Moberger, B., Tryggvason, K. and Auer, G. (1999) J. Natl. Cancer Inst, 91, 1882-1887.

"Expression of the laminin gamma2 chain in different histological types of lung carcinoma. A study by immunohistochemistry and in situ hybridization", Maatta, M., Soini, Y., Paakko, P., Salo, S., Tryggvason, K., Autio-Harmainen, H. (1999) J. Pathol. 188, 361-368.

"Altered distribution and synthesis of laminin-5 (kalinin) in oral lichen planus, epithelial dysplasias and squamous cell carcinomas," Kainulainen, T., Autio-Harmainen, H., Oikarinen, A., Salo, S., Tryggvason, K. and Salo, T. (1997) Br. J. Dermatol. 136, 331.

"Expression of the laminin gamma 2 chain in pancreatic adenocarcinoma," Soini, Y., Maatta, M., Salo, S., Tryggvason, K. and Autio-Harmainen, H. (1996) J. Pathol. 180, 290.

"Laminin-5 is a marker of invading cancer cells in some human carcinomas and is co-expressed with the receptor for urokinase plasminogen activator in budding cancer cells in colon adenocarcinomas," Pyke, C., Salo, S., Ralfkiaer, E., Romer, J., Dano, K. and Tryggvason, K. (1995) Cancer Res. 55, 4132.

"The g2 chain of Kalinin/Laminin-5 is preferentially expressed in invading malignant cells in human cancers," Pyke, C., Romer, J., Kallunki, P., Lund, L.R., Ralfkiaer, E., Dano, K. and Tryggvason, K. (1994) Am. J. Pathol. 145, 782.

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"Primary structure of mouse and rat nephrin cDNA and structure and expression of the mouse gene", Putaala, H., Sainio, K., Sariola, H., Tryggvason, K. (2000) J. Am Soc. Nephrol. 11, 991-1001.

"CD2AP is expressed with nephrin in developing podocytes and is found widely in mature kidney and elsewhere", Li, C., Ruotsalainen, V., Tryggvvason, K., Shaw, A.S., Miner, J.H. (2000) Am J Physiol Renal Physiol 279, F785-792.

"Nephrin is specifically located at the slit diaphragm of glomerular podocytes." Ruotsalainen V, Ljungberg P, Wartiovaara J, Lenkkeri U, Kestila M, Jalanko H, Holmberg C, Tryggvason K (1999) Proc Natl Acad Sci USA 96:7962-7967.

"Glomerular expression of nephrin is decreased in acquired human nephrotic syndrome." Furness PN, Hall LL, Shaw JA, Pringle JH (1999) Nephrol Dial Transplant 14:1234-7.

"Structure of the gene for congenital nephrotic syndrome of the finnish type (NPHS1) and characterization of mutations," Lenkkeri, U., Mannikko, M., McCready, P., Lamerdin, J., Gribouval, O., Niaudet, P.M., Antignac, C.K., Kashtan, C.E., Homberg,C., Olsen, A., Kestila, M. and Tryggvason, K. (1999) Am. J. Hum. Genet. 64, 51-61.

"Unraveling the mechanisms of glomerular ultrafiltration: nephrin, a key component of the slit diaphragm," Tryggvason, K. (1999) J. Am. Soc. Nephrol. 10, 2440-2445.

"Positionally cloned gene for a novel glomerular protein - nephrin- is mutated in congenital nephrotic syndrome," Kestila, M., Lenkkeri, U., Mannikko, M., Lamerdin, J., McCready, P., Putaala, H., Ruotsalainen, V., Morita, T., Nissinen, M., Herva, R., Kashtan, C.E., Peltonen, L., Holmberg, C., Olsen, A., and Tryggvason, K. (1998) Mol. Cell 1, 575-582.

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Glomerular Gene Transfer Therapy

"Animal models of Alport syndrome: advancing the prospects for effective human gene therapy", Heikkila, P., Tryggvason, K., and Thorner, P. (2000) Exp Nephrol, 8, 1-7.

"A novel surgical organ perfusion method for effective ex vivo and in vivo gene transfer into renal glomerular", Parpala-Sparman, T., Lukkarinen, O., Heikkila, P., Tryggvason, K. (1999) Urol Res 27, 97-102.

"Can Alport syndrome be treated by gene therapy," Tryggvason, K., Heikkila, P., Pettersson, E., Tibell, A., Thorner, P. (1997) Kidney Intern. 51, 1493-1499.

"Adenovirus-mediated gene transfer into kidney glomeruli using an ex vivo and in vivo kidney perfusion system - first steps towards gene therapy for Alport Syndrome," Heikkila, Parapala, T., Lukkarinen, O., Weber, M., Tryggvason, K. (1996) Gene Therapy 3, 21-27.

"Identification of mutations in the COL4A5 collagen gene in Alport syndrome," Barker, D., Hostikka, S.L., Zhou, J., Chow, L.T., Oliphant, A.R., Gerken, S.C., Gregory, M.C., Skolnick, M.H., Atkin, C.L. and Tryggvason, K. (1990) Science 248, 1224-1227.

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Second Generation MMP-2 Inhibitors

"Structure of human pro-matrix metalloproteinase-2: activation mechanism revealed," Morgunova E., Tuuttila A., Bergmann U., Isupov M., Lindqvist Y., Schneider G., Tryggvason K. (1999) Science 284, 1667-1670.

"Impaired endochondral ossification and angiogenesis in mice deficient in membrane-type matrix metalloproteinase I", Zhou, Z., Apte, S.S., Soininen, R., Cao, R., Baaklini, G.Y., Rauser, R.W., Wang, J., Cao, Y., Tryggvason, K. (2000) Proc Natl Acad Sci USA, 97, 4052-4057.

"Three-dimensional structure of human tissue inhibitor of metalloproteinases-2 at 2.1 A resolution," Tuuttila, A., Morgunova, E., Bergmann, U., Lindqvist, Y., Maskos, K., Fernandex-Catalan, C., Bode, W., Tryggvason, K., Schneider, G. (1998) J. Mol. Biol. 284, 1133-1140.

"Completion of the primary structure of the human type IV collagenase preproenzyme and assignment of the gene (CLG4) to the q21 region of chromosome 16," Huhtala, P., Eddy, R.L., Fan, Y.S., Byers, M.G., Shows, T.B. and Tryggvason, K. (1990) Genomics 6, 554-559.

"Characterization of gelatinase from pig polymorphonuclear leucosytes. A metalloproteinase resembling tumour type IV collagenase," Murphy, G., Ward, R., Hembry, R.M., Reynolds, J.J., Kuhn, K. and Tryggvason, K. (1989) Biochem. J. 258, 463-472.

"Metastatic potential correlates with enzymatic degradation of basement membrane collagen," Liotta, L.A., Tryggvason, K., Garbisa, S., Foltz, C.M. and Shafie, S. (1980) Nature 284, 67-68.

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Molecular Basis of Autoimmune Disease

"The goodpasture autoantigen. Identification of multiple cryptic epitopes on the NC1 domain of the alpha3(IV) collagen chain", Borza, D.B., Netzer, K.O., Leinonen, A., Todd, P., Cervera, J., Saus, J., and Hudson, B.G. (2000) J. Biol. Chem. 275, 6030-6037.

"GPBP, the kinase that phosphorylates the Goodpasture antigen, is an alternative spliced variant implicated in autoimmune pathologenesis", Raya, A., Revert-Ros, F., Martinez-Martinez, P., Navarro, S., Rosello, E., Vieites, B., Granero, F., Forteza, J., Saus, J. (2000) J. Biol. Chem. Sep 27th.

"Characterization of a novel type of serine/threonine kinase that specifically phosphorylates the human goodpasture antigen", Raya, A., Revert, F., Navarro, S., Saus, J. (1999) J. Biol. Chem. 274, 12642-12649.

"The alpha 3 chain of type IV collagen induces autoimmune Goodpasture syndrome," Kalluri, R., Gattone, V.H., Noelken, M.E. and Hudson, B.G. (1994) Proc. Natl. Acad. Sci. USA 91, 6201-6205.

"Identification of the Goodpasture antigen as the alpha 3(IV) chain of collagen IV," Saus, J., Wieslander, J., Langeveld, J.P., Quinones, S. and Hudson, B.G. (1988) J. Biol. Chem. 263, 13374-13380.

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